Single-cell transcriptomics reveals cell-type-specific diversification in human heart failure

Heart failure represents a major cause of morbidity and mortality worldwide. Single-cell transcriptomics have revolutionized our understanding of cell composition and associated gene expression. Through integrated analysis of single-cell and single-nucleus RNA-sequencing data generated from 27 healthy donors and 18 individuals with dilated cardiomyopathy, here we define the cell composition of the healthy and failing human heart. We identify cell-specific transcriptional signatures associated with age and heart failure and reveal the emergence of disease-associated cell states. Notably, cardiomyocytes converge toward common disease-associated cell states, whereas fibroblasts and myeloid cells undergo dramatic…

• BW
  Burroughs Wellcome Fund

  Award: 1014782
• AH
  American Heart Association
• FF
  Foundation for Barnes-Jewish Hospital

  Award: 8038-88
• CD
  Children's Discovery Institute

  Awards: CDI-CORE-2019-813, PM-LI-2019-829, CH-II-2015-462, CDI-CORE-2015-505
• FL
  Fondation Leducq

  Awards: 20CVD02, #20CVD02
• CF
  Center for Cellular Imaging, Washington University
• NH
  National Heart, Lung, and Blood Institute

  Awards: T32 HL125241, T32 HL134635, HL139714, R01 HL139714, HL151078, HL138466, HL161185, R35 HL161185, R01 HL138466