Single-cell roadmap of human gonadal development

Abstract Gonadal development is a complex process that involves sex determination followed by divergent maturation into either testes or ovaries 1 . Historically, limited tissue accessibility, a lack of reliable in vitro models and critical differences between humans and mice have hampered our knowledge of human gonadogenesis, despite its importance in gonadal conditions and infertility. Here, we generated a comprehensive map of first- and second-trimester human gonads using a combination of single-cell and spatial transcriptomics, chromatin accessibility assays and fluorescent microscopy. We extracted human-specific regulatory programmes that control the development of germline and somatic cell lineages by…

• WT
  Wellcome Trust

  Award: WT206194
• CR
  Cancer Research UK
• NU
  Newcastle University
• EC
  European Commission

  Awards: 874741, 874867, 836291
• MR
  Medical Research Council

  Awards: MR/R006237/1, MR/S036350/1
• BA
  Biotechnology and Biological Sciences Research Council