Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization
Universidad Complutense de Madrid · Technical University of Denmark · +3 more institutions
Abstract
Enzyme immobilization has been developing since the 1960s and although many industrial biocatalytic processes use the technology to improve enzyme performance, still today we are far from full exploitation of the field. One clear reason is that many evaluate immobilization based on only a few experiments that are not always well-designed. In contrast to many other reviews on the subject, here we highlight the pitfalls of using incorrectly designed immobilization protocols and explain why in many cases sub-optimal results are obtained. We also describe solutions to overcome these challenges and come to the conclusion that recent developments in material science, bioprocess engineering and protein science…
Citation impact
- FWCI
- 39.85
- Percentile
- 100%
- References
- 507
Authors
3Topics & keywords
- Bioprocess
- Biochemical engineering
- Subject (documents)
- Computer science
- Nanotechnology
- Immobilized enzyme
- Engineering ethics
- Management science
- Industry, innovation and infrastructure