Metabolic reprogramming from glycolysis to fatty acid uptake and beta-oxidation in platinum-resistant cancer cells

Increased glycolysis is considered as a hallmark of cancer. Yet, cancer cell metabolic reprograming during therapeutic resistance development is under-studied. Here, through high-throughput stimulated Raman scattering imaging and single cell analysis, we find that cisplatin-resistant cells exhibit increased fatty acids (FA) uptake, accompanied by decreased glucose uptake and lipogenesis, indicating reprogramming from glucose to FA dependent anabolic and energy metabolism. A metabolic index incorporating glucose derived anabolism and FA uptake correlates linearly to the level of cisplatin resistance in ovarian cancer (OC) cell lines and primary cells. The increased FA uptake facilitates cancer cell survival…

• NU
  Northwestern University

  Awards: CA060553, NCI CA060553
• NI
  National Institutes of Health

  Awards: S10OD024993, NCI CA060553, R35GM136223, R01CA224275, R33CA223581, CA060553
• NO
  NIH Office of the Director

  Award: S10OD024993
• NC
  National Cancer Institute

  Awards: NCI CA060553, R33CA223581, R01CA224275, CA060553