articleNature CommunicationsAug 6, 2022GOLD OA

A single-cell atlas of the multicellular ecosystem of primary and metastatic hepatocellular carcinoma

Beijing Proteome Research Center · State Key Laboratory of Proteomics · +4 more institutions

PubMed
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Abstract

Abstract Hepatocellular carcinoma (HCC) represents a paradigm of the relation between tumor microenvironment (TME) and tumor development. Here, we generate a single-cell atlas of the multicellular ecosystem of HCC from four tissue sites. We show the enrichment of central memory T cells (T CM ) in the early tertiary lymphoid structures (E-TLSs) in HCC and assess the relationships between chronic HBV/HCV infection and T cell infiltration and exhaustion. We find the MMP9 + macrophages to be terminally differentiated tumor-associated macrophages (TAMs) and PPARγ to be the pivotal transcription factor driving their differentiation. We also characterize the heterogeneous subpopulations of malignant hepatocytes and…

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