Spatial profiling of chromatin accessibility in mouse and human tissues

Deng, Yanxiang; Bartošovič, Marek; Ma, Sai; Zhang, Di; Kukanja, Petra; Xiao, Yang; Su, Graham; Liu, Yang; Qin, Xiaoyu; Rosoklija, Gorazd; Dwork, Andrew J.; Mann, J. John; Xu, Mina L.; Halene, Stephanie; Craft, Joseph; Leong, Kam W.; Boldrini, Maura; Castelo‐Branco, Gonçalo; Fan, Rong

doi:10.1038/s41586-022-05094-1

articleNatureAug 17, 2022HYBRID OA

Spatial profiling of chromatin accessibility in mouse and human tissues

YDYanxiang Deng MBMarek Bartošovič SMSai Ma DZDi Zhang PKPetra Kukanja

Yale Cancer Center · Yale University · +8 more institutions

PubMed

Indexed incrossrefdatacitepubmed

Abstract

Abstract Cellular function in tissue is dependent on the local environment, requiring new methods for spatial mapping of biomolecules and cells in the tissue context 1 . The emergence of spatial transcriptomics has enabled genome-scale gene expression mapping 2–5 , but the ability to capture spatial epigenetic information of tissue at the cellular level and genome scale is lacking. Here we describe a method for spatially resolved chromatin accessibility profiling of tissue sections using next-generation sequencing (spatial-ATAC-seq) by combining in situ Tn5 transposition chemistry 6 and microfluidic deterministic barcoding 5 . Profiling mouse embryos using spatial-ATAC-seq delineated tissue-region-specific…

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350

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Topics & keywords

Topics

Keywords

Chromatin
Profiling (computer programming)
Computational biology
Biology
Cell biology
Computer science
Genetics
DNA

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