Embryo model completes gastrulation to neurulation and organogenesis
California Institute of Technology · University of Padua · +8 more institutions
Abstract
Abstract Embryonic stem (ES) cells can undergo many aspects of mammalian embryogenesis in vitro 1–5 , but their developmental potential is substantially extended by interactions with extraembryonic stem cells, including trophoblast stem (TS) cells, extraembryonic endoderm stem (XEN) cells and inducible XEN (iXEN) cells 6–11 . Here we assembled stem cell-derived embryos in vitro from mouse ES cells, TS cells and iXEN cells and showed that they recapitulate the development of whole natural mouse embryo in utero up to day 8.5 post-fertilization. Our embryo model displays headfolds with defined forebrain and midbrain regions and develops a beating heart-like structure, a trunk comprising a neural tube and somites,…
Citation impact
- FWCI
- 20.70
- Percentile
- 100%
- References
- 91
Authors
15- GAGianluca AmadeiCorresponding
California Institute of Technology, University of Padua, University of Cambridge
- CECharlotte E. Handford
California Institute of Technology, University of Cambridge
- CQChengxiang Qiu
University of Washington
- JDJoachim De Jonghe
University of Cambridge, The Francis Crick Institute
- HGHannah Greenfeld
California Institute of Technology
Topics & keywords
- Biology
- Cell biology
- Neurulation
- Neural tube
- Organogenesis
- Gastrulation
- Neural plate
- Notochord
- Life below water