Interleukin-1β Enhances NMDA Receptor-Mediated Intracellular Calcium Increase through Activation of the Src Family of Kinases
Scripps Research Institute · University of Milan · +1 more institution
Abstract
Interleukin (IL)-1β is a proinflammatory cytokine implicated in various pathophysiological conditions of the CNS involving NMDA receptor activation. Circumstantial evidence suggests that IL-1β and NMDA receptors can functionally interact. Using primary cultures of rat hippocampal neurons, we investigated whether IL-1β affects NMDA receptor function(s) by studying (1) NMDA receptor-induced [Ca 2+ ] i increase and (2) NMDA-mediated neurotoxicity. IL1β (0.01-0.1 ng/ml) dose-dependently enhances NMDA-induced [Ca 2+ ] i increases with a maximal effect of ∼45%. This effect occurred only when neurons were pretreated with IL-1β, whereas it was absent if IL-1β and NMDA were applied simultaneously, and it was abolished…
Citation impact
- FWCI
- 15.95
- Percentile
- 100%
- References
- 39
Authors
11- BVBarbara VivianiCorresponding
Scripps Research Institute, University of Milan, Mario Negri Institute for Pharmacological Research
- SBStefano Bartesaghi
Scripps Research Institute, University of Milan, Mario Negri Institute for Pharmacological Research
- FGFabrizio Gardoni
Scripps Research Institute, University of Milan, Mario Negri Institute for Pharmacological Research
- AVAnnamaria Vezzani
Scripps Research Institute, University of Milan, Mario Negri Institute for Pharmacological Research
- MMM. Margarita Behrens
Scripps Research Institute, University of Milan, Mario Negri Institute for Pharmacological Research
Topics & keywords
- NMDA receptor
- Tyrosine kinase
- Tyrosine phosphorylation
- Biology
- Glutamate receptor
- Proto-oncogene tyrosine-protein kinase Src
- Cell biology
- Receptor
- Good health and well-being