Liquid biopsy: current technology and clinical applications
University of California San Diego · Medical College of Wisconsin · +2 more institutions
Abstract
Liquid biopsies are increasingly used for cancer molecular profiling that enables a precision oncology approach. Circulating extracellular nucleic acids (cell-free DNA; cfDNA), circulating tumor DNA (ctDNA), and circulating tumor cells (CTCs) can be isolated from the blood and other body fluids. This review will focus on current technologies and clinical applications for liquid biopsies. ctDNA/cfDNA has been isolated and analyzed using many techniques, e.g., droplet digital polymerase chain reaction, beads, emulsion, amplification, and magnetics (BEAMing), tagged-amplicon deep sequencing (TAm-Seq), cancer personalized profiling by deep sequencing (CAPP-Seq), whole genome bisulfite sequencing (WGBS-Seq), whole…
Citation impact
- FWCI
- 71.64
- Percentile
- 100%
- References
- 104
Authors
3Topics & keywords
- Liquid biopsy
- Digital polymerase chain reaction
- Circulating tumor cell
- Cell-free fetal DNA
- Amplicon
- Deep sequencing
- Medicine
- Immune checkpoint