HypoMap—a unified single-cell gene expression atlas of the murine hypothalamus

Steuernagel, Lukas; Lam, Brian; Klemm, Paul; Dowsett, Georgina K.C.; Bauder, Corinna A.; Tadross, John A.; Hitschfeld, Tamara Sotelo; Martin, Almudena del Rio; Chen, Wei‐Yi; Solís, Alain J. De; Fenselau, Henning; Davidsen, Peter K.; Cimino, Irène; Kohnke, Sara; Rimmington, Debra; Coll, Anthony P.; Beyer, Andreas; Yeo, Giles S.H.; Brüning, Jens C.

doi:10.1038/s42255-022-00657-y

articleNature MetabolismOct 20, 2022HYBRID OA

HypoMap—a unified single-cell gene expression atlas of the murine hypothalamus

LSLukas Steuernagel BLBrian Lam PKPaul Klemm GKGeorgina K.C. Dowsett CACorinna A. Bauder

Max Planck Institute for Metabolism Research · University of Cambridge · +9 more institutions

PubMed

Indexed incrossrefdatacitepubmed

Abstract

The hypothalamus plays a key role in coordinating fundamental body functions. Despite recent progress in single-cell technologies, a unified catalog and molecular characterization of the heterogeneous cell types and, specifically, neuronal subtypes in this brain region are still lacking. Here, we present an integrated reference atlas, 'HypoMap,' of the murine hypothalamus, consisting of 384,925 cells, with the ability to incorporate new additional experiments. We validate HypoMap by comparing data collected from Smart-Seq+Fluidigm C1 and bulk RNA sequencing of selected neuronal cell types with different degrees of cellular heterogeneity. Finally, via HypoMap, we identify classes of neurons expressing…

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Topics & keywords

Topics

Keywords

Hypothalamus
Biology
Neuroscience
Computational biology
Cell type
Atlas (anatomy)
Cell
Druggability

UN Sustainable Development Goals

Good health and well-being

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