articleCell stem cellDec 1, 2022HYBRID OA

A reference human induced pluripotent stem cell line for large-scale collaborative studies

CBCaroline B. PantazisAYAndrian YangELErika LaraJAJustin A. McDonoughCBCornelis Blauwendraat

National Institutes of Health · National Institute of Neurological Disorders and Stroke · +48 more institutions

PubMed
Indexed incrossrefdatacitepubmed

Abstract

Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between lines makes it challenging to replicate key findings and integrate data across research groups. To address this issue, we sub-cloned candidate human iPSC lines and deeply characterized their genetic properties using whole genome sequencing, their genomic stability upon CRISPR-Cas9-based gene editing, and their phenotypic properties including differentiation to commonly used cell types. These studies identified KOLF2.1J as an all-around well-performing iPSC line. We then shared KOLF2.1J with groups around the world who tested its performance in head-to-head…

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295
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Topics & keywords

Topics
Keywords
  • Biology
  • Induced pluripotent stem cell
  • Computational biology
  • CRISPR
  • Genome editing
  • Phenotype
  • Stem cell
  • Cas9
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