Molecular and spatial signatures of mouse brain aging at single-cell resolution
Howard Hughes Medical Institute · Harvard University · +3 more institutions
Abstract
The diversity and complex organization of cells in the brain have hindered systematic characterization of age-related changes in its cellular and molecular architecture, limiting our ability to understand the mechanisms underlying its functional decline during aging. Here, we generated a high-resolution cell atlas of brain aging within the frontal cortex and striatum using spatially resolved single-cell transcriptomics and quantified changes in gene expression and spatial organization of major cell types in these regions over the mouse lifespan. We observed substantially more pronounced changes in cell state, gene expression, and spatial organization of non-neuronal cells over neurons. Our data revealed…
Citation impact
- FWCI
- 30.80
- Percentile
- 100%
- References
- 76
Authors
5- WEWilliam E. AllenCorresponding
Howard Hughes Medical Institute, Harvard University, Harvard University Press
- TRTimothy R. Blosser
Howard Hughes Medical Institute, Harvard University
- ZAZuri A. Sullivan
Howard Hughes Medical Institute, Harvard University
- CDCatherine DulacCorresponding
Howard Hughes Medical Institute, Harvard University, Harvard University Press, Harvard Stem Cell Institute
- XZXiaowei ZhuangCorresponding
Howard Hughes Medical Institute, Harvard University, Harvard University Press, Harvard Global Health Institute
Topics & keywords
- Biology
- Neuroscience
- Aging brain
- Transcriptome
- Cell
- Gene expression
- Cell type
- Spatial organization