A DNA methylation atlas of normal human cell types

Abstract DNA methylation is a fundamental epigenetic mark that governs gene expression and chromatin organization, thus providing a window into cellular identity and developmental processes 1 . Current datasets typically include only a fraction of methylation sites and are often based either on cell lines that underwent massive changes in culture or on tissues containing unspecified mixtures of cells 2–5 . Here we describe a human methylome atlas, based on deep whole-genome bisulfite sequencing, allowing fragment-level analysis across thousands of unique markers for 39 cell types sorted from 205 healthy tissue samples. Replicates of the same cell type are more than 99.5% identical, demonstrating the robustness…

• LM
  Leona M. and Harry B. Helmsley Charitable Trust
• HM
  Hadassah Medical Organization
• HI
  Human Islet Research Network
• IS
  Israel Science Foundation

  Award: 1250/18
• SD
  Stichting Diabetes Onderzoek Nederland
• L
  Leibniz-Gemeinschaft