articleNatureJan 4, 2023HYBRID OA

Phenotypic signatures of immune selection in HIV-1 reservoir cells

Brigham and Women's Hospital · Ragon Institute of MGH, MIT and Harvard · +4 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

Abstract Human immunodeficiency virus 1 (HIV-1) reservoir cells persist lifelong despite antiretroviral treatment 1,2 but may be vulnerable to host immune responses that could be exploited in strategies to cure HIV-1. Here we used a single-cell, next-generation sequencing approach for the direct ex vivo phenotypic profiling of individual HIV-1-infected memory CD4 + T cells from peripheral blood and lymph nodes of people living with HIV-1 and receiving antiretroviral treatment for approximately 10 years. We demonstrate that in peripheral blood, cells harbouring genome-intact proviruses and large clones of virally infected cells frequently express ensemble signatures of surface markers conferring increased…

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