A transcription factor atlas of directed differentiation

Transcription factors (TFs) regulate gene programs, thereby controlling diverse cellular processes and cell states. To comprehensively understand TFs and the programs they control, we created a barcoded library of all annotated human TF splice isoforms (>3,500) and applied it to build a TF Atlas charting expression profiles of human embryonic stem cells (hESCs) overexpressing each TF at single-cell resolution. We mapped TF-induced expression profiles to reference cell types and validated candidate TFs for generation of diverse cell types, spanning all three germ layers and trophoblasts. Targeted screens with subsets of the library allowed us to create a tailored cellular disease model and integrate mRNA…

• HH
  Howard Hughes Medical Institute
• NI
  National Institutes of Health

  Award: HL141201
• HS
  Harvard Stem Cell Institute
• SC
  Stanley Center for Psychiatric Research, Broad Institute
• KC
  Klarman Cell Observatory, Broad Institute
• MI
  McGovern Institute for Brain Research, Massachusetts Institute of Technology
• NH
  National Human Genome Research Institute