articleScience AdvancesJan 11, 2023GOLD OA

Peptide-guided lipid nanoparticles deliver mRNA to the neural retina of rodents and nonhuman primates

MHMarco Herrera‐BarreraRCRenee C. RyalsMGMilan GautamAJAntony JozićMLMadeleine Landry

Oregon State University · Oregon National Primate Research Center · +1 more institution

PubMed
Indexed incrossrefdoajpubmed

Abstract

Lipid nanoparticle (LNP)-based mRNA delivery holds promise for the treatment of inherited retinal degenerations. Currently, LNP-mediated mRNA delivery is restricted to the retinal pigment epithelium (RPE) and Müller glia. LNPs must overcome ocular barriers to transfect neuronal cells critical for visual phototransduction, the photoreceptors (PRs). We used a combinatorial M13 bacteriophage-based heptameric peptide phage display library for the mining of peptide ligands that target PRs. We identified the most promising peptide candidates resulting from in vivo biopanning. Dye-conjugated peptides showed rapid localization to the PRs. LNPs decorated with the top-performing peptide ligands delivered mRNA to the…

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223
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Authors

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Topics & keywords

Topics
Keywords
  • Biopanning
  • Retina
  • Peptide
  • Messenger RNA
  • Phage display
  • Retinal
  • Retinal pigment epithelium
  • Cell biology
UN Sustainable Development Goals
  • Good health and well-being
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