Quantifying the local adaptive landscape of a nascent bacterial community
University of California, Berkeley · Lawrence Berkeley National Laboratory · +3 more institutions
Abstract
The fitness effects of all possible mutations available to an organism largely shape the dynamics of evolutionary adaptation. Yet, whether and how this adaptive landscape changes over evolutionary times, especially upon ecological diversification and changes in community composition, remains poorly understood. We sought to fill this gap by analyzing a stable community of two closely related ecotypes ("L" and "S") shortly after they emerged within the E. coli Long-Term Evolution Experiment (LTEE). We engineered genome-wide barcoded transposon libraries to measure the invasion fitness effects of all possible gene knockouts in the coexisting strains as well as their ancestor, for many different, ecologically…
Citation impact
- FWCI
- 127.18
- Percentile
- 100%
- References
- 74
Authors
5- JAJoao A Ascensao
University of California, Berkeley
- KMKelly M. Wetmore
Lawrence Berkeley National Laboratory
- BHBenjamin H. Good
Stanford University
- APAdam P. Arkin
Lawrence Berkeley National Laboratory, University of California, Berkeley
- OHOskar HallatschekCorresponding
Acupuncture & Integrative Medicine College, Leipzig University, University of California, Berkeley
Topics & keywords
- Fitness landscape
- Epistasis
- Biology
- Adaptation (eye)
- Genetic Fitness
- Local adaptation
- Evolutionary biology
- Ecotype
- Life in Land
Funding
- NSNational Science FoundationAwards: 1555330, CAREER, DE-AC02-05CH11231
- UDU.S. Department of EnergyAwards: -AC02-05CH11231, 05CH11231, AC02-05CH11231, DE-AC02, DE-AC02-05CH11231, DE-AC02-
- APAlfred P. Sloan FoundationAwards: FG-2021-15708, DE-AC02-05CH11231
- ACAdolph C. and Mary Sprague Miller Institute for Basic Research in Science, University of California Berkeley
- NENational Energy Research Scientific Computing CenterAwards: 05CH11231, AC02-05CH11231
- OOOffice of ScienceAwards: AC02-05CH11231, -AC02-05CH11231, DE-AC02
- LBLawrence Berkeley National LaboratoryAwards: DE-AC02-05CH11231, 05CH11231, AC02-05CH11231