articleCellJan 1, 2023HYBRID OA

Multiplexed 3D atlas of state transitions and immune interaction in colorectal cancer

Harvard University · Center for Systems Biology · +2 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

Advanced solid cancers are complex assemblies of tumor, immune, and stromal cells characterized by high intratumoral variation. We use highly multiplexed tissue imaging, 3D reconstruction, spatial statistics, and machine learning to identify cell types and states underlying morphological features of known diagnostic and prognostic significance in colorectal cancer. Quantitation of these features in high-plex marker space reveals recurrent transitions from one tumor morphology to the next, some of which are coincident with long-range gradients in the expression of oncogenes and epigenetic regulators. At the tumor invasive margin, where tumor, normal, and immune cells compete, T cell suppression involves…

No related works found for this paper.

Funding