Exon architecture controls mRNA m 6 A suppression and gene expression
Howard Hughes Medical Institute · University of Chicago · +1 more institution
Abstract
N 6 -methyladenosine (m 6 A) is the most abundant messenger RNA (mRNA) modification and plays crucial roles in diverse physiological processes. Using a massively parallel assay for m 6 A (MPm 6 A), we discover that m 6 A specificity is globally regulated by suppressors that prevent m 6 A deposition in unmethylated transcriptome regions. We identify exon junction complexes (EJCs) as m 6 A suppressors that protect exon junction–proximal RNA within coding sequences from methylation and regulate mRNA stability through m 6 A suppression. EJC suppression of m 6 A underlies multiple global characteristics of mRNA m 6 A specificity, with the local range of EJC protection sufficient to suppress m 6 A deposition in…
Citation impact
- FWCI
- 36.32
- Percentile
- 100%
- References
- 78
Authors
14- PCP. Cody HeCorresponding
Howard Hughes Medical Institute, University of Chicago
- JWJiangbo WeiCorresponding
Howard Hughes Medical Institute, University of Chicago
- XDXiaoyang DouCorresponding
Howard Hughes Medical Institute, University of Chicago
- BTBryan T. HaradaCorresponding
Howard Hughes Medical Institute, University of Chicago
- ZZZijie Zhang
Howard Hughes Medical Institute, Yunnan University, University of Chicago
Topics & keywords
- Exon
- Messenger RNA
- Biology
- RNA
- Nonsense-mediated decay
- Molecular biology
- Intron
- Gene