A compendium of single extracellular vesicle flow cytometry

Welsh, Joshua A; Arkesteijn, Ger J. A.; Bremer, Michel; Cimorelli, Michael; Dignat‐George, Françoise; Giebel, Bernd; Görgens, André; Hendrix, An; Kuiper, Martine; Lacroix, Romaric; Lannigan, Joanne; Leeuwen, Ton G. van; Lozano−Andrés, Estefanía; Rao, Shoaib; Robert, Stéphane; Rond, Leonie de; Tang, Vera A.; Tertel, Tobias; Yan, Xiaomei; Wauben, Marca H. M.; Nolan, John P.; Jones, Jennifer; Nieuwland, Rienk; Pol, Edwin van der

doi:10.1002/jev2.12299

reviewJournal of Extracellular VesiclesFeb 1, 2023GOLD OA

A compendium of single extracellular vesicle flow cytometry

JAJoshua A Welsh GJGer J. A. Arkesteijn MBMichel Bremer MCMichael Cimorelli FDFrançoise Dignat‐George

National Institutes of Health · National Cancer Institute · +25 more institutions

PubMed

Indexed incrossrefdoajpubmed

Abstract

Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells, which are larger than EVs. Whereas cells exceed the background noise, signals originating from EVs partly overlap with the background noise, thereby making EVs more difficult to detect than cells. This technical mismatch together with complexity of EV-containing fluids causes limitations and challenges with conducting, interpreting and reproducing EV FCM experiments. To address and overcome these challenges, researchers from the International Society for Extracellular Vesicles (ISEV), International Society for Advancement of…

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Keywords

Compendium
Extracellular vesicles
Flow cytometry
Cytometry
Computer science
Extracellular vesicle
Reproducibility
Analyte

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