Genome-wide subcellular protein map for the flagellate parasite Trypanosoma brucei

Trypanosoma brucei is a model trypanosomatid, an important group of human, animal and plant unicellular parasites. Understanding their complex cell architecture and life cycle is challenging because, as with most eukaryotic microbes, ~50% of genome-encoded proteins have completely unknown functions. Here, using fluorescence microscopy and cell lines expressing endogenously tagged proteins, we mapped the subcellular localization of 89% of the T. brucei proteome, a resource we call TrypTag. We provide clues to function and define lineage-specific organelle adaptations for parasitism, mapping the ultraconserved cellular architecture of eukaryotes, including the first comprehensive 'cartographic' analysis of the…

• W
  Wellcome

  Awards: 103261/Z/13/Z, 218288/Z/19/Z, 108445/Z/15/Z, 211075/Z/18/Z, 104627/Z/14/Z, 217138/Z/19/Z
• WT
  Wellcome Trust

  Awards: 217138/Z/19/Z, 218288/Z/19/Z, 104627/Z/14/Z, 211075, /Z/15/Z, 103261/Z/13/Z, 211075/Z/18/Z, 108445/Z/15/Z