High cell density and high-resolution 3D bioprinting for fabricating vascularized tissues

Three-dimensional (3D) bioprinting techniques have emerged as the most popular methods to fabricate 3D-engineered tissues; however, there are challenges in simultaneously satisfying the requirements of high cell density (HCD), high cell viability, and fine fabrication resolution. In particular, bioprinting resolution of digital light processing-based 3D bioprinting suffers with increasing bioink cell density due to light scattering. We developed a novel approach to mitigate this scattering-induced deterioration of bioprinting resolution. The inclusion of iodixanol in the bioink enables a 10-fold reduction in light scattering and a substantial improvement in fabrication resolution for bioinks with an HCD.…

• NS
  National Science Foundation

  Awards: 2135720, EB021857, P30 NS047101, NS047101
• NI
  National Institutes of Health

  Awards: CA253615, NS047101, P30 NS047101, EB021857
• UO
  University of California, San Diego

  Awards: P30 NS047101, NS047101
• SO
  School of Medicine, University of California, San Diego

  Award: P30 NS047101