High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq

Liu, Yang; DiStasio, Marcello; Su, Graham; Asashima, Hiromitsu; Enninful, Archibald; Qin, Xiaoyu; Deng, Yanxiang; Nam, Jungmin; Gao, Fu; Bordignon, Pino; Cassano, Marco; Tomayko, Mary M.; Xu, Mina L.; Halene, Stephanie; Craft, Joseph; Hafler, David A.; Fan, Rong

doi:10.1038/s41587-023-01676-0

articleNature BiotechnologyFeb 23, 2023HYBRID OA

High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq

YLYang Liu MDMarcello DiStasio GSGraham Su HAHiromitsu Asashima AEArchibald Enninful

Yale Cancer Center · Yale University

PubMed

Indexed incrossrefpubmed

Abstract

In this study, we extended co-indexing of transcriptomes and epitopes (CITE) to the spatial dimension and demonstrated high-plex protein and whole transcriptome co-mapping. We profiled 189 proteins and whole transcriptome in multiple mouse tissue types with spatial CITE sequencing and then further applied the method to measure 273 proteins and transcriptome in human tissues, revealing spatially distinct germinal center reactions in tonsil and early immune activation in skin at the Coronavirus Disease 2019 mRNA vaccine injection site.

Citation impact

356

total citations

FWCI: 51.02
Percentile: 100%
References: 28

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Authors

17

Topics & keywords

Topics

Keywords

Transcriptome
Biology
Computational biology
De novo transcriptome assembly
Genetics
Gene
Gene expression

UN Sustainable Development Goals

Good health and well-being

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