Transcription shapes 3D chromatin organization by interacting with loop extrusion

Banigan, Edward J.; Tang, Wen; Berg, Aafke A. van den; Stocsits, Roman R.; Wutz, Gordana; Brandão, Hugo B.; Busslinger, Georg; Peters, Jan‐Michael; Mirny, Leonid A.

doi:10.1073/pnas.2210480120

articleProceedings of the National Academy of SciencesMar 10, 2023GREEN OA

Transcription shapes 3D chromatin organization by interacting with loop extrusion

EJEdward J. Banigan WTWen Tang AAAafke A. van den Berg RRRoman R. Stocsits GWGordana Wutz

Massachusetts Institute of Technology · Research Institute of Molecular Pathology · +4 more institutions

PubMed

Indexed incrossrefpubmed

Abstract

Cohesin folds mammalian interphase chromosomes by extruding the chromatin fiber into numerous loops. "Loop extrusion" can be impeded by chromatin-bound factors, such as CTCF, which generates characteristic and functional chromatin organization patterns. It has been proposed that transcription relocalizes or interferes with cohesin and that active promoters are cohesin loading sites. However, the effects of transcription on cohesin have not been reconciled with observations of active extrusion by cohesin. To determine how transcription modulates extrusion, we studied mouse cells in which we could alter cohesin abundance, dynamics, and localization by genetic "knockouts" of the cohesin regulators CTCF and Wapl.…

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Topics & keywords

Topics

Keywords

Cohesin
CTCF
Chromatin
Biology
Transcription (linguistics)
Promoter
Cell biology
Transcription factor

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