A sampling strategy for genome sequencing the British terrestrial arthropod fauna
Osaka Science Museum · University of Oxford · +5 more institutions
Abstract
The Darwin Tree of Life (DToL) project aims to sequence and assemble high-quality genomes from all eukaryote species in Britain and Ireland, with the first phase of the project concentrating on family-level coverage plus species of particular ecological, biomedical or evolutionary interest. We summarise the processes involved in (1) assessing the UK arthropod fauna and the status of individual species on UK lists; (2) prioritising and collecting species for initial genome sequencing; (3) handling methods to ensure that high-quality genomic DNA is preserved; and (4) compiling standard operating procedures for processing specimens for genome sequencing, identification verification and voucher specimen curation.…
Citation impact
- FWCI
- 202.96
- Percentile
- 100%
- References
- 12
Authors
17- LMLiam M CrowleyCorresponding
Osaka Science Museum, University of Oxford, Osaka University of Pharmaceutical Sciences
- HAHeather Allen
Natural History Museum, Osaka Science Museum
- IBIan Barnes
Natural History Museum, Osaka Science Museum
- DBDouglas Boyes
Osaka Science Museum, University of Oxford, UK Centre for Ecology & Hydrology, Osaka University of Pharmaceutical Sciences
- GRGavin R. Broad
Natural History Museum, Osaka Science Museum
Topics & keywords
- Fauna
- Genome
- Biology
- Arthropod
- Identification (biology)
- Evolutionary biology
- DNA sequencing
- Reference genome