Surface Ligand Engineering Ruthenium Nanozyme Superior to Horseradish Peroxidase for Enhanced Immunoassay
Chinese Academy of Sciences · Institute of Biophysics · +4 more institutions
Abstract
Abstract Nanozymes have great potential to be used as an alternative to natural enzymes in a variety of fields. However, low catalytic activity compared with natural enzymes limits their practical use. It is still challenging to design nanozymes comparable to their natural counterparts in terms of the specific activity. In this study, a surface engineering strategy is employed to improve the specific activity of Ru nanozymes using charge‐transferrable ligands such as polystyrene sulfonate (PSS). PSS‐modified Ru nanozyme exhibits a peroxidase‐like specific activity of up to 2820 U mg −1 , which is twice that of horseradish peroxidase (1305 U mg −1 ). Mechanism studies suggest that PSS readily accepts negative…
Citation impact
- FWCI
- 17.88
- Percentile
- 100%
- References
- 52
Authors
8- HFHuizhen Fan
Chinese Academy of Sciences, Institute of Biophysics
- JZJia‐Jia Zheng
Chinese Academy of Sciences, National Center for Nanoscience and Technology
- JXJiaying Xie
Chinese Academy of Sciences, Institute of Biophysics
- JLJuewen Liu
University of Waterloo
- XGXingfa Gao
Chinese Academy of Sciences, National Center for Nanoscience and Technology
Topics & keywords
- Horseradish peroxidase
- Ruthenium
- Materials science
- Immunoassay
- Ligand (biochemistry)
- Surface modification
- Nanotechnology
- Chemical engineering
Funding
- IRInnovative Research Group Project of the National Natural Science Foundation of ChinaAwards: 81930050, 22121003
- NNNational Natural Science Foundation of ChinaAwards: 81930050, 22121003, 82122037, 31900981
- CAChinese Academy of SciencesAwards: JCTD-2020-08, 2019093
- YIYouth Innovation Promotion Association of the Chinese Academy of SciencesAward: 2019093
- NKNational Key Research and Development Program of ChinaAwards: 2021YFC2102900, 2019YFA0709200