articleScienceApr 27, 2023GREEN OA

Integrating gene annotation with orthology inference at scale

Goethe University Frankfurt · Senckenberg Research Institute and Natural History Museum Frankfurt/M · +7 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

Annotating coding genes and inferring orthologs are two classical challenges in genomics and evolutionary biology that have traditionally been approached separately, limiting scalability. We present TOGA (Tool to infer Orthologs from Genome Alignments), a method that integrates structural gene annotation and orthology inference. TOGA implements a different paradigm to infer orthologous loci, improves ortholog detection and annotation of conserved genes compared with state-of-the-art methods, and handles even highly fragmented assemblies. TOGA scales to hundreds of genomes, which we demonstrate by applying it to 488 placental mammal and 501 bird assemblies, creating the largest comparative gene resources so…

Citation impact

197
total citations
FWCI
28.54
Percentile
100%
References
68
Citations per year

Authors

116

Topics & keywords

Keywords
  • Annotation
  • Genome
  • Inference
  • Biology
  • Gene
  • Gene Annotation
  • Computational biology
  • Genomics
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