Applications of fluorescence spectroscopy in protein conformational changes and intermolecular contacts

Rodrigues, Fabio Henrique dos Santos; Delgado, Gonzalo García; Costa, Thyerre Santana da; Tasić, Ljubica

doi:10.1016/j.bbadva.2023.100091

articleBBA AdvancesJan 1, 2023GOLD OA

Applications of fluorescence spectroscopy in protein conformational changes and intermolecular contacts

FHFabio Henrique dos Santos Rodrigues GGGonzalo García Delgado TSThyerre Santana da Costa LTLjubica Tasić

University of Warwick · Universidade Estadual de Campinas (UNICAMP)

PubMed

Indexed incrossrefdoajpubmed

Abstract

Emission fluorescence is one of the most versatile and powerful biophysical techniques used in several scientific subjects. It is extensively applied in the studies of proteins, their conformations, and intermolecular contacts, such as in protein-ligand and protein-protein interactions, allowing qualitative, quantitative, and structural data elucidation. This review, aimed to outline some of the most widely used fluorescence techniques in this area, illustrate their applications and display a few examples. At first, the data on the intrinsic fluorescence of proteins is disclosed, mainly on the tryptophan side chain. Predominantly, research to study protein conformational changes, protein interactions, and…

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Topics

Keywords

Fluorescence
Fluorescence anisotropy
Fluorophore
Chemistry
Intermolecular force
Population
Fluorescence spectroscopy
Fluorescence in the life sciences

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