CRISPR-Cas13a-powered electrochemical biosensor for the detection of the L452R mutation in clinical samples of SARS-CoV-2 variants

Since the end of 2019, a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has deprived numerous lives worldwide, called COVID-19. Up to date, omicron is the latest variant of concern, and BA.5 is replacing the BA.2 variant to become the main subtype rampaging worldwide. These subtypes harbor an L452R mutation, which increases their transmissibility among vaccinated people. Current methods for identifying SARS-CoV-2 variants are mainly based on polymerase chain reaction (PCR) followed by gene sequencing, making time-consuming processes and expensive instrumentation indispensable. In this study, we developed a rapid and ultrasensitive electrochemical biosensor to…

• GM
  Guangzhou Medical University

  Award: GZMU-SH-001
• NN
  National Natural Science Foundation of China

  Awards: 12274197, 81870337
• GO
  Government of Jiangsu Province
• KA
  King Abdulaziz University

  Awards: FP-158-43, No. FP-158-43
• NS
  Natural Science Foundation of Jiangsu Province

  Award: BK20210076
• KK
  King Khalid University

  Award: RCAMS/KKU/006/21
• GM
  Guizhou Minzu University
• SU
  Shenzhen University
• DO
  Department of Education of Guangdong Province

  Award: 2018KCXTD026
• ST
  Science, Technology and Innovation Commission of Shenzhen Municipality

  Awards: KCXFZ20201221173413038, JSGG20200225152648408, JCYJ20220818102618040