articleMolecular CancerMay 9, 2023GOLD OA

Aberrant m5C hypermethylation mediates intrinsic resistance to gefitinib through NSUN2/YBX1/QSOX1 axis in EGFR-mutant non-small-cell lung cancer

YWYueqin WangJWJingyao WeiLFLuyao FengOLOuwen LiLHLan Huang

Zhengzhou University · First Affiliated Hospital of Zhengzhou University · +2 more institutions

PubMed
Indexed incrossrefdoajpubmed

Abstract

Abstract Background RNA 5-methylcytosine (m 5 C) modification plays critical roles in the pathogenesis of various tumors. However, the function and molecular mechanism of RNA m 5 C modification in tumor drug resistance remain unclear. Methods The correlation between RNA m 5 C methylation, m 5 C writer NOP2/Sun RNA methyltransferase family member 2 (NSUN2) and EGFR-TKIs resistance was determined in non-small-cell lung cancer (NSCLC) cell lines and patient samples. The effects of NSUN2 on EGFR-TKIs resistance were investigated by gain- and loss-of-function assays in vitro and in vivo . RNA-sequencing (RNA-seq), RNA bisulfite sequencing (RNA-BisSeq) and m 5 C methylated RNA immunoprecipitation-qPCR (MeRIP-qPCR)…

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207
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30.67
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Authors

20

Topics & keywords

Topics
Keywords
  • Gefitinib
  • Biology
  • Cancer research
  • Lung cancer
  • Mutant
  • Lung
  • Cancer
  • Methylation
UN Sustainable Development Goals
  • Good health and well-being
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