Profiling the human intestinal environment under physiological conditions

Abstract The spatiotemporal structure of the human microbiome 1,2 , proteome 3 and metabolome 4,5 reflects and determines regional intestinal physiology and may have implications for disease 6 . Yet, little is known about the distribution of microorganisms, their environment and their biochemical activity in the gut because of reliance on stool samples and limited access to only some regions of the gut using endoscopy in fasting or sedated individuals 7 . To address these deficiencies, we developed an ingestible device that collects samples from multiple regions of the human intestinal tract during normal digestion. Collection of 240 intestinal samples from 15 healthy individuals using the device and…

• NS
  National Science Foundation

  Award: 1936687
• BA
  Bill and Melinda Gates Foundation

  Award: INV-037857
• FF
  Fund for Astrophysical Research
• NI
  National Institutes of Health

  Awards: GM135102, DK007056, NIH T32, RM1 GM135102, AI147023
• SO
  School of Medicine, Stanford University
• ND
  National Defense Science and Engineering Graduate
• SR
  Stanford Research Computing Center, Stanford University