Single-cell multi-omics in the medicinal plant Catharanthus roseus

Advances in omics technologies now permit the generation of highly contiguous genome assemblies, detection of transcripts and metabolites at the level of single cells and high-resolution determination of gene regulatory features. Here, using a complementary, multi-omics approach, we interrogated the monoterpene indole alkaloid (MIA) biosynthetic pathway in Catharanthus roseus, a source of leading anticancer drugs. We identified clusters of genes involved in MIA biosynthesis on the eight C. roseus chromosomes and extensive gene duplication of MIA pathway genes. Clustering was not limited to the linear genome, and through chromatin interaction data, MIA pathway genes were present within the same topologically…

• UO
  University of Georgia
• MS
  Michigan State University
• GR
  Georgia Research Alliance
• M
  Max-Planck-Gesellschaft
• CO
  College of Engineering, Michigan State University
• JS
  Japan Society for the Promotion of Science

  Award: 20J00973