articleNatureMay 24, 2023HYBRID OA

Ångström-resolution fluorescence microscopy

SRS. ReinhardtLALuciano A. MasulloIPIsabelle PachmayrPRPhilipp R. SteenRKRafal Kowalewski

Center for NanoScience · Max Planck Institute of Biochemistry · +3 more institutions

PubMed
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Abstract

Abstract Fluorescence microscopy, with its molecular specificity, is one of the major characterization methods used in the life sciences to understand complex biological systems. Super-resolution approaches 1–6 can achieve resolution in cells in the range of 15 to 20 nm, but interactions between individual biomolecules occur at length scales below 10 nm and characterization of intramolecular structure requires Ångström resolution. State-of-the-art super-resolution implementations 7–14 have demonstrated spatial resolutions down to 5 nm and localization precisions of 1 nm under certain in vitro conditions. However, such resolutions do not directly translate to experiments in cells, and Ångström resolution has…

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Topics & keywords

Topics
Keywords
  • Resolution (logic)
  • Microscopy
  • Biomolecule
  • Fluorescence microscope
  • Angstrom
  • Fluorescence
  • Biological specimen
  • Characterization (materials science)
UN Sustainable Development Goals
  • Responsible consumption and production
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