articleHorticulture ResearchJun 13, 2023GOLD OA

quarTeT: a telomere-to-telomere toolkit for gap-free genome assembly and centromeric repeat identification

Anhui Agricultural University · Sichuan University · +3 more institutions

PubMed
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Abstract

Abstract A high-quality genome is the basis for studies on functional, evolutionary, and comparative genomics. The majority of attention has been paid to the solution of complex chromosome structures and highly repetitive sequences, along with the emergence of a new ‘telomere-to-telomere (T2T) assembly’ era. However, the bioinformatic tools for the automatic construction and/or characterization of T2T genome are limited. Here, we developed a user-friendly web toolkit, quarTeT, which currently includes four modules: AssemblyMapper, GapFiller, TeloExplorer, and CentroMiner. First, AssemblyMapper is designed to assemble phased contigs into the chromosome-level genome by referring to a closely related genome.…

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