Single-cell and spatial transcriptome analysis reveals the cellular heterogeneity of liver metastatic colorectal cancer
Guangdong Academy of Medical Sciences · Guangdong Provincial People's Hospital · +5 more institutions
Abstract
In this study, we comprehensively charted the cellular landscape of colorectal cancer (CRC) and well-matched liver metastatic CRC using single-cell and spatial transcriptome RNA sequencing. We generated 41,892 CD45 − nonimmune cells and 196,473 CD45 + immune cells from 27 samples of six CRC patients, and found that CD8_CXCL13 and CD4_CXCL13 subsets increased significantly in liver metastatic samples that exhibited high proliferation ability and tumor-activating characterization, contributing to better prognosis of patients. Distinct fibroblast profiles were observed in primary and liver metastatic tumors. F3 + fibroblasts enriched in primary tumors contributed to worse overall survival by expressing protumor…
Citation impact
- FWCI
- 43.62
- Percentile
- 100%
- References
- 56
Authors
13- FWFei WangCorresponding
Guangdong Academy of Medical Sciences, Guangdong Provincial People's Hospital, Southern Medical University
- JLJie LongCorresponding
Guangdong Academy of Medical Sciences, Guangdong Provincial People's Hospital, Southern Medical University
- LLLiang Li
Guangdong Academy of Medical Sciences, Guangdong Provincial People's Hospital, Southern Medical University
- ZWZixin Wu
Guangzhou First People's Hospital, Guangzhou Medical University
- TDTian-Tian Da
South China University of Technology
Topics & keywords
- Transcriptome
- Colorectal cancer
- Metastasis
- Cancer research
- Biology
- CD8
- Cell
- Cancer
- Good health and well-being