Spatial transcriptomics: Technologies, applications and experimental considerations
Qingdao University · China Medical University · +7 more institutions
Abstract
The diverse cell types of an organ have a highly structured organization to enable their efficient and correct function. To fully appreciate gene functions in a given cell type, one needs to understand how much, when and where the gene is expressed. Classic bulk RNA sequencing and popular single cell sequencing destroy cell structural organization and fail to provide spatial information. However, the spatial location of gene expression or of the cell in a complex tissue provides key clues to comprehend how the neighboring genes or cells cross talk, transduce signals and work together as a team to complete the job. The functional requirement for the spatial content has been a driving force for rapid development…
Citation impact
- FWCI
- 25.64
- Percentile
- 100%
- References
- 63
Authors
9- YWYe WangCorresponding
Qingdao University
- BLBin Liu
China Medical University, Liaoning Cancer Hospital & Institute
- GZGexin Zhao
UCLA Medical Center
- YLYoojin Lee
UCLA Medical Center
- ABAnton Buzdin
Institute of Physics and Technology, Sechenov University, Moscow Institute of Physics and Technology, Institute of Bioorganic Chemistry
Topics & keywords
- Biology
- Computational biology
- Transcriptome
- Spatial organization
- Function (biology)
- Focus (optics)
- Gene
- Spatial analysis
Funding
- QKQingdao Key Health Discipline Development Fund
- NNNational Natural Science Foundation of ChinaAward: 81670822
- MOMinistry of Education and Science of the Russian Federation
- MOMinistry of Science and Higher Education of the Russian FederationAwards: 075-15-2022-304, 075-15-2022, 075-15-
- NINational Institutes of Health