Discovery of deaminase functions by structure-based protein clustering
Chinese Academy of Sciences · Institute of Genetics and Developmental Biology · +8 more institutions
Abstract
The elucidation of protein function and its exploitation in bioengineering have greatly advanced the life sciences. Protein mining efforts generally rely on amino acid sequences rather than protein structures. We describe here the use of AlphaFold2 to predict and subsequently cluster an entire protein family based on predicted structure similarities. We selected deaminase proteins to analyze and identified many previously unknown properties. We were surprised to find that most proteins in the DddA-like clade were not double-stranded DNA deaminases. We engineered the smallest single-strand-specific cytidine deaminase, enabling efficient cytosine base editor (CBE) to be packaged into a single adeno-associated…
Citation impact
- FWCI
- 32.60
- Percentile
- 100%
- References
- 68
Authors
20- JHJiaying Huang
Chinese Academy of Sciences, Institute of Genetics and Developmental Biology
- QLQiupeng Lin
Chinese Academy of Sciences, Institute of Genetics and Developmental Biology
- HFHongyuan Fei
Chinese Academy of Sciences, State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, University of Chinese Academy of Sciences
- ZHZixin He
Chinese Academy of Sciences, State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, University of Chinese Academy of Sciences
- HXHu Xu
Topics & keywords
- Biology
- Cytosine deaminase
- Cytidine deaminase
- Computational biology
- DNA
- Cytosine
- Genetics
- Protein structure
- Zero hunger