Spatially resolved single-cell translatomics at molecular resolution
Broad Institute · Massachusetts Institute of Technology
Abstract
The precise control of messenger RNA (mRNA) translation is a crucial step in posttranscriptional gene regulation of cellular physiology. However, it remains a challenge to systematically study mRNA translation at the transcriptomic scale with spatial and single-cell resolution. Here, we report the development of ribosome-bound mRNA mapping (RIBOmap), a highly multiplexed three-dimensional in situ profiling method to detect cellular translatome. RIBOmap profiling of 981 genes in HeLa cells revealed cell cycle-dependent translational control and colocalized translation of functional gene modules. We mapped 5413 genes in mouse brain tissues, yielding spatially resolved single-cell translatomic profiles for…
Citation impact
- FWCI
- 26.75
- Percentile
- 100%
- References
- 69
Authors
13- HZHu ZengCorresponding
Broad Institute, Massachusetts Institute of Technology
- JHJiahao HuangCorresponding
Broad Institute, Massachusetts Institute of Technology
- JRJingyi RenCorresponding
Broad Institute, Massachusetts Institute of Technology
- CKConnie Kangni Wang
Broad Institute
- ZTZefang Tang
Broad Institute, Massachusetts Institute of Technology
Topics & keywords
- Ribosome profiling
- Translation (biology)
- Biology
- Messenger RNA
- Transcriptome
- Cell biology
- Gene expression
- Internal ribosome entry site