Spatially resolved multiomics of human cardiac niches

Abstract The function of a cell is defined by its intrinsic characteristics and its niche: the tissue microenvironment in which it dwells. Here we combine single-cell and spatial transcriptomics data to discover cellular niches within eight regions of the human heart. We map cells to microanatomical locations and integrate knowledge-based and unsupervised structural annotations. We also profile the cells of the human cardiac conduction system 1 . The results revealed their distinctive repertoire of ion channels, G-protein-coupled receptors (GPCRs) and regulatory networks, and implicated FOXP2 in the pacemaker phenotype. We show that the sinoatrial node is compartmentalized, with a core of pacemaker cells,…

• TS
  Takeda Science Foundation
• NB
  NHS Blood and Transplant

  Award: NIHR203332
• WT
  Wellcome Trust

  Award: WT206194
• DZ
  Deutsches Zentrum für Herz-Kreislaufforschung

  Award: SP/19/1/34461
• BS
  British Society for Heart Failure
• NI
  National Institute for Health and Care Research

  Award: NIHR203332
• BH
  British Heart Foundation

  Award: FS/CRTF/23/24444
• DO
  Department of Health and Social Care
• IC
  Imperial College London
• IC
  Imperial College Healthcare NHS Trust
• NU
  Newcastle University
• EC
  European Commission
• DF
  Deutsche Forschungsgemeinschaft

  Award: SFB-1470
• FL
  Fondation Leducq

  Award: 16CVD03
• CS
  China Scholarship Council
• NI
  NIHR Imperial Biomedical Research Centre