The Origin of Highly Elevated Cell-Free DNA in Healthy Individuals and Patients with Pancreatic, Colorectal, Lung, or Ovarian Cancer
Howard Hughes Medical Institute · Johns Hopkins University · +10 more institutions
Abstract
Cell-free DNA (cfDNA) concentrations from patients with cancer are often elevated compared with those of healthy controls, but the sources of this extra cfDNA have never been determined. To address this issue, we assessed cfDNA methylation patterns in 178 patients with cancers of the colon, pancreas, lung, or ovary and 64 patients without cancer. Eighty-three of these individuals had cfDNA concentrations much greater than those generally observed in healthy subjects. The major contributor of cfDNA in all samples was leukocytes, accounting for ∼76% of cfDNA, with neutrophils predominating. This was true regardless of whether the samples were derived from patients with cancer or the total plasma cfDNA…
Citation impact
- FWCI
- 40.85
- Percentile
- 100%
- References
- 98
Authors
23- AKAustin K. Mattox
Howard Hughes Medical Institute, Johns Hopkins University, Johns Hopkins Medicine, Sidney Kimmel Comprehensive Cancer Center
- CDChristopher Douville
Howard Hughes Medical Institute, Johns Hopkins University, Johns Hopkins Medicine, Sidney Kimmel Comprehensive Cancer Center
- YWYuxuan Wang
Howard Hughes Medical Institute, Johns Hopkins University, Johns Hopkins Medicine, Sidney Kimmel Comprehensive Cancer Center
- MPMaria Popoli
Johns Hopkins University, Johns Hopkins Medicine, Sidney Kimmel Comprehensive Cancer Center
- JPJanine Ptak
Howard Hughes Medical Institute, Johns Hopkins University, Johns Hopkins Medicine, Sidney Kimmel Comprehensive Cancer Center
Topics & keywords
- Colorectal cancer
- Lung
- Medicine
- Cancer research
- Oncology
- Cell
- Internal medicine
- DNA
- Good health and well-being