Assessment of Four Engineered PET Degrading Enzymes Considering Large-Scale Industrial Applications

In recent years, enzymatic recycling of the widely used polyester polyethylene terephthalate (PET) has become a complementary solution to current thermomechanical recycling for colored, opaque, and mixed PET. A large set of promising hydrolases that depolymerize PET have been found and enhanced by worldwide initiatives using various methods of protein engineering. Despite the achievements made in these works, it remains difficult to compare enzymes’ performance and their applicability to large-scale reactions due to a lack of homogeneity between the experimental protocols used. Here, we pave the way for a standardized enzymatic PET hydrolysis protocol using reaction conditions relevant for larger scale…

• IE
  Infrastructures en Biologie Santé et Agronomie
• AD
  Agence de l'Environnement et de la Maîtrise de l'Energie

  Award: 1882C0098
• H2
  Horizon 2020 Framework Programme

  Awards: 870294, 953214
• HB
  Helmholtz-Zentrum Berlin für Materialien und Energie