Lactobacillus gallinarum -derived metabolites boost anti-PD1 efficacy in colorectal cancer by inhibiting regulatory T cells through modulating IDO1/Kyn/AHR axis
Chinese University of Hong Kong · Institut de Recherche en Santé Digestive · +4 more institutions
Abstract
Objective Gut microbiota is a key player in dictating immunotherapy response. We aimed to explore the immunomodulatory effect of probiotic Lactobacillus gallinarum and its role in improving anti-programmed cell death protein 1 (PD1) efficacy against colorectal cancer (CRC). Design The effects of L. gallinarum in anti-PD1 response were assessed in syngeneic mouse models and azoxymethane/dextran sulfate sodium-induced CRC model. The change of immune landscape was identified by multicolour flow cytometry and validated by immunohistochemistry staining and in vitro functional assays. Liquid chromatography-mass spectrometry was performed to identify the functional metabolites. Results L. gallinarum significantly…
Citation impact
- FWCI
- 23.69
- Percentile
- 100%
- References
- 46
Authors
13- WFWinnie Fong
Chinese University of Hong Kong, Institut de Recherche en Santé Digestive
- QLQing Li
Chinese University of Hong Kong, Institut de Recherche en Santé Digestive
- FJFenfen Ji
Chinese University of Hong Kong, Institut de Recherche en Santé Digestive
- WLWei Liang
Sun Yat-sen University, Institute of Precision Mechanics, The First Affiliated Hospital, Sun Yat-sen University
- HCHarry Cheuk-Hay Lau
Chinese University of Hong Kong, Institut de Recherche en Santé Digestive
Topics & keywords
- FOXP3
- Cancer research
- Colorectal cancer
- Regulatory T cell
- Biology
- Immune system
- Flow cytometry
- T cell
- Good health and well-being