preprintSep 11, 2023GOLD OA
Sanger Tree of Life HMW DNA Fragmentation: Diagenode Megaruptor®3 for PacBio HiFi v1
Indexed incrossref
Abstract
This protocol describes the fragmentation of HMW DNA from either the MagAttract v.1, Plant MagAttract v.1, or Plant MagAttract v.2 Sanger Tree of Life HMW DNA extraction protocols, using the Diagenode Megaruptor®3. This process is highly effective for DNA extracts from all of the taxonomic groups covered by the Tree of Life Programme, with DNA sheared into an average fragment size range of 12–20 kb. However, challenging samples include those that are highly concentrated or highly viscous, as well as samples which contain contaminants or impurities following DNA extraction. The output of this protocol is sheared DNA which can be directed towards fragmented DNA clean up, with either the Manual or Automated SPRI…
Citation impact
284
total citations
- FWCI
- 83.03
- Percentile
- 100%
- References
- 0
Citations per year
Authors
3- MTMaja TodorovicCorresponding
Wellcome Sanger Institute
- FSFilipa Sampaio
- CHCaroline Howard
Topics & keywords
Topics
Keywords
- DNA
- Sanger sequencing
- DNA extraction
- Fragmentation (computing)
- DNA fragmentation
- Computational biology
- Tree (set theory)
- Biology
No related works found for this paper.