articleScienceOct 12, 2023GREEN OA

Single-cell analysis of prenatal and postnatal human cortical development

DVDmitry VelmeshevYPYonatan PerezZYZihan YanJEJonathan E. ValenciaDRDavid R. Castañeda-Castellanos

Duke University · University of California, San Francisco · +12 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

We analyzed >700,000 single-nucleus RNA sequencing profiles from 106 donors during prenatal and postnatal developmental stages and identified lineage-specific programs that underlie the development of specific subtypes of excitatory cortical neurons, interneurons, glial cell types, and brain vasculature. By leveraging single-nucleus chromatin accessibility data, we delineated enhancer gene regulatory networks and transcription factors that control commitment of specific cortical lineages. By intersecting our results with genetic risk factors for human brain diseases, we identified the cortical cell types and lineages most vulnerable to genetic insults of different brain disorders, especially autism. We find…

Citation impact

174
total citations
FWCI
25.29
Percentile
100%
References
37
Citations per year

Authors

14

Topics & keywords

Topics
Keywords
  • Biology
  • Autism
  • Enhancer
  • Chromatin
  • Neuroscience
  • Lineage (genetic)
  • Cell type
  • Transcription factor
No related works found for this paper.

Funding