NULISA: a proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing

The blood proteome holds great promise for precision medicine but poses substantial challenges due to the low abundance of most plasma proteins and the vast dynamic range of the plasma proteome. Here we address these challenges with NUcleic acid Linked Immuno-Sandwich Assay (NULISA™), which improves the sensitivity of traditional proximity ligation assays by ~10,000-fold to attomolar level, by suppressing assay background via a dual capture and release mechanism built into oligonucleotide-conjugated antibodies. Highly multiplexed quantification of both low- and high-abundance proteins spanning a wide dynamic range is achieved by attenuating signals from abundant targets with unconjugated antibodies and…

• SB
  Small Business Innovation Research
• DF
  Deutsche Forschungsgemeinschaft

  Awards: EXC 2151, IRTG2168, 272482170, Excellence Cluster EXC 2151 (ID: 390873048), IRTG2168 (ID: 272482170), 390873048
• BF
  Bundesministerium für Bildung und Forschung

  Award: 01KI20343
• NC
  National Cancer Institute

  Award: 1R43CA254548-01