Dictionary of immune responses to cytokines at single-cell resolution

, yet we lack a global view of the cellular responses of each immune cell type to each cytokine. To address this gap, we created the Immune Dictionary, a compendium of single-cell transcriptomic profiles of more than 17 immune cell types in response to each of 86 cytokines (>1,400 cytokine-cell type combinations) in mouse lymph nodes in vivo. A cytokine-centric view of the dictionary revealed that most cytokines induce highly cell-type-specific responses. For example, the inflammatory cytokine interleukin-1β induces distinct gene programmes in almost every cell type. A cell-type-centric view of the dictionary identified more than 66 cytokine-driven cellular polarization states across immune cell types,…

• DM
  Dr. Miriam and Sheldon G. Adelson Medical Research Foundation
• BI
  Broad Institute
• NI
  National Institutes of Health

  Award: RM1HG006193
• NS
  Natural Sciences and Engineering Research Council of Canada
• NC
  National Cancer Institute

  Award: R50CA251956