articleNature BiotechnologyJan 11, 2024HYBRID OA

In vivo human T cell engineering with enveloped delivery vehicles

JHJennifer HamiltonECEvelyn ChenBSBarbara S. PerezCRCindy R. Sandoval EspinozaMHMin Hyung Kang

Innovative Genomics Institute · Century Therapeutics (United States) · +5 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

Viruses and virally derived particles have the intrinsic capacity to deliver molecules to cells, but the difficulty of readily altering cell-type selectivity has hindered their use for therapeutic delivery. Here, we show that cell surface marker recognition by antibody fragments displayed on membrane-derived particles encapsulating CRISPR-Cas9 protein and guide RNA can deliver genome editing tools to specific cells. Compared to conventional vectors like adeno-associated virus that rely on evolved capsid tropisms to deliver virally encoded cargo, these Cas9-packaging enveloped delivery vehicles (Cas9-EDVs) leverage predictable antibody-antigen interactions to transiently deliver genome editing machinery…

Citation impact

220
total citations
FWCI
45.83
Percentile
100%
References
54
Citations per year

Authors

8

Topics & keywords

Topics
Keywords
  • Genome editing
  • Cas9
  • CRISPR
  • Biology
  • Gene delivery
  • Genome engineering
  • Cell biology
  • Chimeric antigen receptor
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