articleScienceJan 18, 2024HYBRID OA

Priming agents transiently reduce the clearance of cell-free DNA to improve liquid biopsies

CMCarmen Martin-AlonsoSTShervin TabriziKXKan XiongTBTimothy BlewettSSSainetra Sridhar

Harvard–MIT Division of Health Sciences and Technology · Massachusetts Institute of Technology · +8 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

Liquid biopsies enable early detection and monitoring of diseases such as cancer, but their sensitivity remains limited by the scarcity of analytes such as cell-free DNA (cfDNA) in blood. Improvements to sensitivity have primarily relied on enhancing sequencing technology ex vivo. We sought to transiently augment the level of circulating tumor DNA (ctDNA) in a blood draw by attenuating its clearance in vivo. We report two intravenous priming agents given 1 to 2 hours before a blood draw to recover more ctDNA. Our priming agents consist of nanoparticles that act on the cells responsible for cfDNA clearance and DNA-binding antibodies that protect cfDNA. In tumor-bearing mice, they greatly increase the recovery…

Citation impact

157
total citations
FWCI
40.79
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100%
References
78
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Authors

21

Topics & keywords

Topics
Keywords
  • Priming (agriculture)
  • Ex vivo
  • In vivo
  • Liquid biopsy
  • Cell-free fetal DNA
  • DNA
  • Circulating tumor DNA
  • Cancer research
UN Sustainable Development Goals
  • Good health and well-being
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