Microglia regulate sleep through calcium-dependent modulation of norepinephrine transmission
Howard Hughes Medical Institute · University of California, Berkeley · +6 more institutions
Abstract
Abstract Sleep interacts reciprocally with immune system activity, but its specific relationship with microglia—the resident immune cells in the brain—remains poorly understood. Here, we show in mice that microglia can regulate sleep through a mechanism involving G i -coupled GPCRs, intracellular Ca 2+ signaling and suppression of norepinephrine transmission. Chemogenetic activation of microglia G i signaling strongly promoted sleep, whereas pharmacological blockade of G i -coupled P2Y12 receptors decreased sleep. Two-photon imaging in the cortex showed that P2Y12–G i activation elevated microglia intracellular Ca 2+ , and blockade of this Ca 2+ elevation largely abolished the G i -induced sleep increase.…
Citation impact
- FWCI
- 23.82
- Percentile
- 100%
- References
- 72
Authors
17- CMChenyan MaCorresponding
Howard Hughes Medical Institute, University of California, Berkeley
- BLBing Li
Howard Hughes Medical Institute, University of California, Berkeley
- DSDaniel Silverman
Howard Hughes Medical Institute, University of California, Berkeley
- XDXinlu Ding
Howard Hughes Medical Institute, University of California, Berkeley
- ALAnan Li
Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou Institute of Biomedical Engineering and Technology, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology
Topics & keywords
- Microglia
- Norepinephrine
- Neuroscience
- Adenosine
- Internal medicine
- Endocrinology
- Blockade
- Chemistry