Development of Better Aptamers: Structured Library Approaches, Selection Methods, and Chemical Modifications
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Abstract
Systematic evolution of ligands by exponential enrichment (SELEX) has been used to discover thousands of aptamers since its development in 1990. Aptamers are short single-stranded oligonucleotides capable of binding to targets with high specificity and selectivity through structural recognition. While aptamers offer advantages over other molecular recognition elements such as their ease of production, smaller size, extended shelf-life, and lower immunogenicity, they have yet to show significant success in real-world applications. By analyzing the importance of structured library designs, reviewing different SELEX methodologies, and the effects of chemical modifications, we provide a comprehensive overview on…
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144
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Authors
5Topics & keywords
Topics
Keywords
- Aptamer
- Systematic evolution of ligands by exponential enrichment
- Oligonucleotide
- Computational biology
- Computer science
- Nanotechnology
- Selection (genetic algorithm)
- Molecular recognition
UN Sustainable Development Goals
- Good health and well-being
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